High-Speed Genome Scanning Analysis Based on Automated Detection and Matching Spots in Autoradiogram Images of 2-D Gel Electrophoresis
نویسندگان
چکیده
In this paper, we present the pattern analysis of autoradiogram images obtained in two-dimensional gel electrophoresis based on the RLGS (restriction landmark genome scanning) method [1]. On a RLGS autoradiogram image, several thousands of spots can be observed; one spot corresponds to one genetic locus and its intensity re ects the copy number of the restriction landmark. In the rst step of the computer analysis, geometrical location and density of each spot was detected by applying some kinds of operators, including gradient and ring operators, to the autoradiogram image. As the second step, structured features of the spot pattern was extracted and represented as structured graph. Two or more structured graphs can be matched and compared by computer e ciently, enabling high-speed and fully-automated scanning of genomic DNA.
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